• Species Reactivity

  Mouse

• Specificity

  Detects mouse TIM-3 in direct ELISAs and Western blots.

• Source

  Monoclonal Rat IgG    _2A Clone # 215008

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse TIM‑3    
  Arg20-Ala193    
  Accession # Q8VIM0

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1 - 3). There are three TIM genes in human and eight in mouse. Mature mouse TIM-3 consists of a 174 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 67 aa cytoplasmic tail (4). Two alternately spliced isoforms have been reported in mouse which lack either the TM or both the TM and mucin regions (5, 6). Within the ECD, mouse TIM-3 shares 58% and 74% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is specifically expressed on Th1 cells whereas TIM-1 and TIM-2 are expressed on TH2 cells. In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types and on hippocampal neurons (9 - 12). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9 which induces TIM-3 Tyr phosphorylation and proapoptotic signaling(10, 13). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation, and cytotoxicity (5, 7, 12, 14). TIM-3 may play a role in regulatory T cell development, (7) inflammation, (15) and immune tolerance (5, 13, 14). Soluble mouse TIM-3 has been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (6, 7).

• References:

1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.

2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.

3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.

4. Monney, L. et al. (2002) Nature 415:536.

5. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.

6. Geng, H. et al. (2006) J. Immunol. 176:1411.

7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.

8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

9. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.

10. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.

11. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.

12. Oikawa, T. et al. (2006) J. Immunol. 177:4281.

13. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.

14. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.

15. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.

• Long Name:

  T Cell Immunoglobulin Mucin-3

• Entrez Gene IDs:

  84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)

• Alternate Names:

  CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD-3; TIMD3KIM-3