• Species Reactivity

  Human, Mouse

• Specificity

  Detects human Semaphorin 3A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Semaphorin 3B, 3E, 6A, 6B, 6C, 6D, 7A, recombinant mouse Semaphorin 3C, or 3F is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 215803

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3A    
  Lys26-Val771 (Arg555Ala, Arg552Ala)    
  Accession # Q14563

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 3A

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).

Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2-like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1‑3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

• References:

1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.

2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.

3. Kolodkin, A.L. et al. (1993) Cell 75:1389.

4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.

5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.

6. Luo Y. et al. (1993) Cell 75:217.

• Entrez Gene IDs:

  10371 (Human); 102126862 (Cynomolgus Monkey)

• Alternate Names:

  (semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD