• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of immobilized rmEDAR/Fc Chimera to bind biotinylated rmEDA-A1 in a functional ELISA.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse EDAR (Glu27 - Ile189) Accession # Q9R187	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu27

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  44.3 kDa (monomer)

• SDS-PAGE

  55-60 kDa, reducing conditions

Background: EDAR

EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region, the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Mouse EDAR is a 488 amino acid (aa) protein with a predicted 30 aa signal, a 159 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The human and mouse EDAR homologs share 91% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2 which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds to EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

• References:

1. Headon, D.J. and Overbeek, P.A. (1999) Nat. Genet. 22:370.

2. Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.

3. Monreal, A.W. et al. (1999) Nat. Genet. 22:366.

4. Schneider, P. et al. (2001) J.  Biol. Chem. 276:18819.

5. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.

6. Yan, M. et al. (2000) Science 209:523.

• Long Name:

  Ectodysplasin Receptor

• Entrez Gene IDs:

  10913 (Human); 13608 (Mouse)

• Alternate Names:

  Anhidrotic ectodysplasin receptor 1; DLED3; Ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; ectodysplasin A receptor; Ectodysplasin-A receptor; ED1R; ED5; EDA1R; EDA3EDA-A1 receptor; EDA-A1R; EDAR; FLJ94390; HRM1; mouse, homolog of; tumor necrosis factor receptor superfamily member EDAR