• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED    ₅₀ for this effect is 0.3-1.5 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse RELT/TNFRSF19L (Met1-Gln169) Accession # Q8BX43	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Thr32

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  41.6 kDa (monomer)

• SDS-PAGE

  40-55 kDa, reducing condtions

Background: RELT/TNFRSF19L

RELT (Receptor Expressed in Lymphoid Tissues) is a 46 kDa (predicted) type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily and designated TNFRSF19‑like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes (2, 3). Mouse RELT cDNA encodes 436 amino acids (aa), including a 31 aa signal peptide, a 138 aa extracellular domain (ECD) containing a TNF receptor cysteine‑rich domain and a potential N‑linked glycosylation site, a 21 aa transmembrane domain, and a 246 aa cytoplasmic region that lacks a death domain (2). Within the ECD, mouse RELT shares 78%, 89%, 70%, 70% and 67% aa sequence homology with human, rat, canine, porcine and bovine RELT, respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TROY/TNFRSF19 and OX40 (2). Neither human nor mouse RELT bind any of the ~19 TNF superfamily ligands that have been tested (1). Two related transmembrane proteins, RELL1 and RELL2, have been identified in both human and mouse (3). RELL1 and 2 are coexpressed with and can interact with RELT, and are thought to modulate its signaling (3‑5). Intracellularly, RELT has been shown to bind the adaptor protein TRAF‑1 and activate the NF‑ kappa B pathway, the phospholipid scramblase PLSCR1, and the oxidative stress responsive protein OSR1 (2‑5). Another investigator notes association and signaling through SPAK, but not TRAF or NF kappa B (6). When overexpressed in HEK‑293 cells, RELT induces p38 and JNK signaling and activates apoptosis (4‑6).

• References:

1. Bossen C. et al. (2006) J. Biol. Chem. 281:13964.

2. Sica, G. et al. (2001) Blood 97:2702.

3. Cusick, J.K. et al. (2006) Biochem. Biophys. Res. Commun. 340:535.

4. Cusick, J.K. et al. (2010) Cell. Immunol. 261:1.

5. Cusick, J.K. et al. (2012) Mol. Cell. Biochem. 362:55.

6. Polek T.C. et al. (2006) Biochem. Biophys. Res. Commun. 343:125.

• Long Name:

  Receptor Expressed in Lymphoid Tissues

• Entrez Gene IDs:

  84957 (Human); 320100 (Mouse)

• Alternate Names:

  Receptor expressed in lymphoid tissues; RELT tumor necrosis factor receptor; RELT; TNFRSF19L; TNFRSF19LFLJ14993; tumor necrosis factor receptor superfamily member 19L; tumor necrosis factor receptor superfamily, member 19-like