• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind Recombinant Mouse DDR2 Fc Chimera with an apparent K    _D <10 nM.    
  Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse DDR2 (Gln24-Arg399) Accession # NP_072075	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Gln24 predicted: No results obtained, sequencing might be blocked

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  69.6 kDa (monomer)

• SDS-PAGE

  80-90 kDa, reducing conditions

Background: DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin‑like domain containing subfamily of receptor tyrosine kinases (1). Mature mouse DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 433 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, mouse DDR2 shares 50% aa sequence identity with DDR1. It shares 97% and 99% aa sequence identity with human and rat DDR2, respectively. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3‑5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding. The D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a noncovalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces up‑regulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). It promotes osteoblast and chondrocyte differentiation and supports ovulation and spermatogenesis (11‑13). DDR2 is up‑regulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell overgrowth diseases (8, 10, 14, 15).

• References:

1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.

2. Karn, T. et al. (1993) Oncogene 8:3433.

3. Vogel, W. et al. (1997) Mol. Cell 1:13.

4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.

5. Leitinger, B. and Kwan, A.P.L. (2006) Matrix Biol. 25:355.

6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.

7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.

8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.

9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.

10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.

11. Zhang, Y. et al. (2011) J. Bone Miner. Res. 26:604.

12. Matsumura, H. et al. (2009) Physiol. Genomics 39:120.

13. Kano, K. et al. (2010) Mol. Reprod. Dev. 77:29.

14. Wang, J. et al. (2002) J. Autoimmun. 19:161.

15. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

• Long Name:

  Discoidin Domain Receptor 2

• Entrez Gene IDs:

  4921 (Human); 18214 (Mouse)

• Alternate Names:

  CD167 antigen-like family member B; CD167b antigen; DDR2; Discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain receptor tyrosine kinase 2; discoidin domain-containing receptor 2; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3cell migration-inducing protein 20; Receptor protein-tyrosine kinase TKT; TKT; TKTMIG20a; Trk3; TYRO10; Tyro-10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase