• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 1.9-15 μg/mL on a nitrocellulose-coated microplate.

• Source

  Mouse myeloma cell line, NS0-derived Gln19-Pro910, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln19 predicted, sequencing might be blocked

• Structure / Form

  Noncovalently-linked homodimer

• Predicted Molecular Mass

  99.8 kDa (monomer)

• SDS-PAGE

  115-125 kDa, reducing conditions

Background: MDGA1

MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1) is a 135-140 kDa glycoprotein within the IgCAM superfamily of adhesion molecules (1-3). MDGA1 is thought to mediate neuronal migration, neurite outgrowth, and cell-cell adhesion within the central and peripheral nervous system (1, 4-8). Mouse MDGA1 precursor is a 940 amino acid (aa) protein that produces an 899 aa mature protein with six Ig-like domains, a fibronectin type III domain (aa 622-724), a MAM domain (aa 736-903), and a GPI anchor (aa 917) (1-3). Mature mouse MDGA1 shares 95%, 97%, and 94% aa sequence identity with mature human, rat, and canine MDGA1, respectively. Mouse MDGA1 also shares 52% aa sequence identity with mouse MDGA2, and may also share some functional redundancy (1, 4). MDGA1 is mainly expressed on restricted populations of neurons in the central and peripheral nervous system, such as embryonic neurons destined for cortical layers 2/3, migrating basilar pontine neurons and D1 interneurons of the spinal cord (1, 5-7). Deletion or down‑regulation of mouse MDGA1 slows radial migration of neurons, indicating a role for MDGA1 in radial migration of cortical neurons (4, 5). MAM and Ig-like domains are involved in heterophilic and homophilic adhesion (7, 8). MDGA1 expression is also reported on primary cells and cell lines from leukemias, lymphomas, and tumors of the lung, colon, uterus, stomach and breast (3).

• References:

1. Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.

2. Diaz-Lopez, A. et al. (2005) Exp. Cell Res. 307:91.

3. De Juan, C. et al. (2002) Oncogene 21:3089.

4. Ishikawa, T. et al. (2011) Dev. Dyn. 240:96.

5. Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.

6. Takeuchi, A. et al. (2007) Cereb Cortex. 17:1531.

7. Fujimura, Y. et al. (2006) Brain Res. 1101:12.

8. Diaz-Lopez, A. et al. (2011) Cancer Microenviron. 4:23.

• Long Name:

  MAM Domain Containing Glycosylphosphatidylinositol Anchor 1

• Entrez Gene IDs:

  266727 (Human); 74762 (Mouse)

• Alternate Names:

  FLJ45018; GPIM; MAM domain containing glycosylphosphatidylinositol anchor 1; MAMDC3; MDGA1