• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED    ₅₀ for this effect is 2‑6 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # ) and 1 µg/mL of recombinant mouse TSG.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse Chordin (Thr27-Ser948) Accession # Q9Z0E2	IEGR	GGGSGGGSGGGS	10-His tag
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Thr27

• Predicted Molecular Mass

  101.5 kDa

• SDS-PAGE

  100-115 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Chordin

Chordin is a secreted glycoprotein that regulates dorsoventral patterning during gastrulation. Chordin functions as a bone morphogenetic protein (BMP) antagonist that blocks their ventralizing activity by binding to the BMPs and inhibiting their interaction with their receptors. Mouse Chordin cDNA encodes a 948 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide. Chordin contains four internal cysteine-rich repeats (CRs) that are conserved in the spacing of their ten cysteine residues. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding. These conserved CRs are present in an expanding family of secreted molecules that antagonize BMP signaling. Xolloid (an extracellular zinc metalloproteinase) can cleave chordin at two specific sites resulting in chordin fragments with lower BMP-affinity. Cleavage of the chordin/BMP complex can reverse the BMP antagonist activity of chordin. Mouse chordin is expressed at high levels in 7 day postcoitum mouse embryos. Chordin expression is also detected in multiple fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles for chordin in organogenesis and homeostasis.

• References:

1. De Robertis, E.M. and Y. Sasai (1996) Nature 380:37.

2. Larrain, J. et al. (2000) Development 127:821.

3. Coffinier, C. et al. (2001) Mech. Dev. 100:119.

• Entrez Gene IDs:

  8646 (Human); 12667 (Mouse)

• Alternate Names:

  Chordin; CHRD; MGC133038; Sog