• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED    ₅₀ for this effect is 0.75-3.5 μg/mL.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Mouse CD300E (Leu18-Leu166) Accession # EDL34457	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Leu18

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  43.6 kDa (monomer)

• SDS-PAGE

  55-60 kDa, reducing conditions

Background: CD300e/LMIR6

CD300E, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35 kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 154 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 4 aa cytoplasmic stub (2). Within the ECD, mouse CD300E shares 57% and 78% aa sequence identity with human and rat CD300E, respectively. CD300E is expressed on monocytes and dendritic cells (3, 4). Its transmembrane segment contains a charged lysine residue which mediates CD300E association with the signaling adaptor molecule DAP12 and enables CD300E to function as an activating receptor (2, 3). Antibody crosslinking of CD300E promotes monocyte and dendritic cell survival, production of inflammatory chemokines and cytokines, upregulation of costimulatory molecules, and also the ability of dendritic cells to induce T cell proliferation (4).

• References:

1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.

2. Chung, D.H. et al. (2003) J. Immunol. 141:6541.

3. Aguilar, H. et al. (2004) J. Immunol. 173:6703.

4. Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.

• Entrez Gene IDs:

  342510 (Human); 217306 (Mouse)

• Alternate Names:

  CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2