详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Human Vitronectin (Catalog # ) is coated at 1 μg/mL, Recombinant Mouse Integrin alpha V beta 5 binds with an apparent K D <0.5 nM.
- SourceChinese Hamster Ovary cell line, CHO-derived
Mouse Integrin alpha V
(Phe31-Val988)
Accession # P43406HP GS Linker Acidic Tail HHHHHH Mouse Integrin beta 5
(Gly24-Asn719)
Accession # NP_001139356HP GS Linker Basic Tail N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisPhe31 ( alpha V subunit) & Gly24 ( beta 5 subunit) - Structure / FormNoncovalently-linked heterodimer
- Predicted Molecular Mass115 kDa ( alpha V subunit) & 84.8 kDa ( beta 5 subunit)
- SDS-PAGE140-155 kDa & 105-115 kDa, reducing conditions
| 7706-AV | | |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
| Reconstitution Reconstitute at 300 μg/mL in PBS. | ||
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Integrin alpha V beta 5 is one of five alpha V integrins and the only known beta 5 integrin (1‑3). The non‑covalent heterodimer of 170 kDa alpha V and 100‑110 kDa beta 5 integrin type I transmembrane glycoprotein subunits is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium (4‑8). alpha V beta 5 binds ligands containing an RGD motif, notably vitronectin (1‑10). The 958 aa mouse alpha V extracellular domain (ECD) shares 92‑95% aa sequence identity with human and bovine alpha V, while the 696 aa mouse beta 5 ECD shares 97%, 91% and 91% aa sequence identity with rat, human and bovine beta 5, respectively. The alpha V ECD contains an N‑terminal beta ‑propeller structure, followed by domains termed thigh, calf‑1 and calf‑2 (1). The 799 aa beta 5 contains a vWFA domain within the ECD, which interacts with the alpha V beta ‑propeller to form a binding domain. Each subunit has a transmembrane sequence and a short cytoplasmic tail. Potential beta 5 isoforms include a 691 aa form with an alternate start site at aa 109, a 958 aa form with an alternate N‑terminus, and a 795 aa form with an alternate C‑terminus. Post‑translational modifications, such as proteolytic cleavage of the alpha V subunit or phosphorylation of the beta 5 cytoplasmic tail, can increase endocytic turnover of the alpha V beta 5 protein and/or promote cell migration (7‑10). Growth factors that increase PKC activity, such as VEGF or TGF‑ alpha, promote alpha V beta 5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF‑basic and TNF‑ alpha (11). An inhibitor of both down‑regulates tumor angiogenesis (12). During lung inflammation, up‑regulation of alpha V beta 5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF‑ beta from latency (13, 14). On retinal pigment epithelia, alpha V beta 5 is important for normal diurnal phagocytosis of outer rod segments, and contributes to adhesion of retinal cells (15).
- References:
- Hynes, R.O. (2002) Cell 110:673.
- Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
- Suzuki, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5354.
- Smith, J.W. et al. (1990) J. Biol. Chem. 265:11008.
- Pasqualini, R. et al. (1993) J. Cell Sci. 105:101.
- Braam, S.R. et al. (2008) Stem Cells 26:2257.
- Memmo, L.M. and P. McKeown-Longo (1998) J. Cell Sci. 111:425.
- Kim, J.P. et al. (1994) J. Biol. Chem. 269:26926.
- Berthet, V. et al. (2004) FEBS Lett. 557:159.
- Li, Z. et al. (2010) Mol. Biol. Cell 21:3317.
- Friedlander, M. et al. (1995) Science 270:1500.
- Desgrosellier, J.S. and D.A. Cheresh (2010) Nat. Rev. Cancer 10:9.
- Wipff, P.J. et al. (2007) J. Cell Biol. 179:1311.
- Luzina, I.G. et al. (2009) Arthritis Rheum. 60:1530.
- Nandrot, E.F. et al. (2004) J. Exp. Med. 200:1539.
- Entrez Gene IDs:3685 (Human)
- Alternate Names:Integrin alpha V beta 5
400-6226-992