• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Bovine Fibronectin (Catalog # ) is coated at 2 μg/mL, Recombinant Mouse Integrin alpha 5 beta 1 binds with an apparent K    _D <0.5 nM.   

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Mouse Integrin alpha 5 Phe45-Asn999 (Gly377Ala) Accession # P11688	GS Linker	Acidic Tail	HHHHHH
  Mouse Integrin beta 1 (Gln21-Asp728) Accession # P09055	HP	GS Linker	Basic Tail
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Phe45 (Integrin alpha 5) & Gln21 predicted, No results obtained: sequencing might be blocked (Integrin beta 1)

• Structure / Form

  Noncovalently-linked heterodimer 

• Predicted Molecular Mass

  113 kDa (Integrin alpha 5) & 86.5 kDa (Integrin beta 1)

• SDS-PAGE

  115-165 kDa, reducing conditions

Background: Integrin alpha 5 beta 1

Integrin alpha 5 beta 1, also known as VLA‑5, is a widely expressed non‑covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). Mature mouse Integrin  alpha 5/CD49e consists of a 955 aa extracellular domain (ECD) with 7 tandem FG‑GAP repeats, a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain (3). Within the ECD, mouse alpha 5 shares 91% and 97% aa sequence identity with human and rat alpha 5, respectively. Mature mouse Integrin beta 1/CD29 consists of a 708 aa ECD with one vWF‑A domain and a cysteine‑rich region, followed by a 23 aa transmembrane segment and a 47 aa cytoplasmic domain (3). Within the ECD, mouse beta 1 shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. alpha 5/ beta 1 binds Fibronectin in both RGD‑dependent and ‑independent manners (4, 5). It is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association   in cis of alpha 5/ beta 1 with VEGF R3 and Tie‑2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including ErbB2, uPAR, Galectin‑1, CTGF, and thrombin‑cleaved Osteopontin (5, 10‑13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14‑16).

• References:

1. Mao, Y. and Schwarzbauer, J.E. (2005) Matrix Biol. 24:389.

2. Gu, J. and Taniguchi, N. (2004) Glycoconj. J. 21:9.

3. Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.

4. Takagi, J. et al. (2003) EMBO J. 22:4607.

5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.

6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.

7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.

8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.

9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.

10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.

11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.

12. Gao, R. and Brigstock, D.R. (2005) Gastroenterology 129:1019.

13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.

14. Carstanjen, D. et al. (2005) Transfusion 45:1192.

15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.

16. Tanaka, R. et al. (2009) J. Biol. Chem. 284:19817.

• Entrez Gene IDs:

  3678 (Human)

• Alternate Names:

  Integrin alpha 5 beta 1; VLA-5