详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Human Laminin alpha 4 (Catalog # ) is coated at 5 μg/mL, Recombinant Mouse Integrin alpha 6 beta 1 binds with an apparent K D <5nM.
- SourceChinese Hamster Ovary cell line, CHO-derived
Mouse Integrin alpha 6
(Phe24-Gly1011)
Accession # Q61739HP GGGSGGGS Acidic Tail HHHHHH Mouse Integrin beta 1
(Gln21-Asp728)
Accession # P09055HP GGGSGGGS Basic Tail N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisPhe24 ( alpha 6 subunit) & Gln21 predicted: No results obtained, sequencing might be blocked ( beta 1 subunit) - Structure / FormNoncovalently-linked heterodimer
- Predicted Molecular Mass119 kDa ( alpha 6 subunit) & 86.4 kDa ( beta 1 subunit)
- SDS-PAGE105-120 & 125-150 kDa, reducing conditions
| 7810-A6 | | |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
| Reconstitution Reconstitute at 250 μg/mL in PBS. | ||
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Integrin alpha 6 beta 1
Integrin alpha 6 beta 1, also called platelet glycoprotein GPIc-IIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (1-9). The non-covalent heterodimer is composed of ~150 kDa alpha 6/CD49f and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits (2). While alpha 6 pairs only with beta 1 or beta 4, twelve integrins share the beta 1 subunit (1‑5). The alpha 6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha 6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1, 2). The mouse alpha 6 heavy chain shares 98% aa identity with rat and 92‑93% with human (X1), bovine, and canine alpha 6, and the mouse beta 1 ECD shares 98% aa identity with rat and 93‑94% with human, bovine, porcine, ovine, canine and feline beta 1. alpha 6 beta 1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and self-renewal on LN‑511 (laminin alpha 5 beta 1 gamma 1) (6, 7). The secreted protein Netrin‑4 and the laminin gamma 1 subunit form an adhesion‑activating complex with alpha 6 beta 1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha 6 beta 1 up‑regulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (12‑14). alpha 6 beta 1 cleavage via uPA (urokinase‑type plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha 6 beta 1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13).
- References:
- Takada, Y. et al. (2007) Genome Biol. 8:215.
- Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
- Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.
- Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
- Sonnenberg, A. and C.J.T. Linders (1990) J. Cell Science 96:207.
- Rodin, S. et al. (2010) Nat. Biotech. 28:611.
- Domogatskaya A. et al. (2008) Stem Cells 26:2800.
- Staquicini, F.I. et al. (2009) Proc. Natl. Acad. Sci. USA 106:2903.
- Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770.
- Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
- Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
- Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.
- Dai, J.Y. et al. (2009) BMC Cancer 9:337.
- Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.
- Alternate Names:Integrin alpha 6 beta 1
400-6226-992