• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to enhance anti-CD3-induced proliferation of mouse CD3    ⁺ T cells. The ED    ₅₀ for this effect is 0.25‑1.25 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse SECTM1A (Gln28-Thr165) Accession # NP_663348	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Gln28 predicted: No results obtained, sequencing might be blocked

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  42.6 kDa (monomer)

• SDS-PAGE

  55-66 kDa, reducing conditions

Background: SECTM1A

SECTM1A (secreted and transmembrane 1A), is 192 amino acid (aa) protein that shares approximately 45‑51%, 81%, and 43% aa sequence identity with human SECTM1 (also called K12), rat SECTM1, and mouse SECTM1B/K12, respectively, and appears to share structural and functional characteristics with other SECTM1 proteins. Human SECTM1 can be found either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form, and is produced by some myeloid cells and by thymic epithelia and fibroblasts (1‑3). Stimulation with IFN‑ gamma is often necessary to detect human SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Mouse SECTM1A cDNA encodes a signal sequence, an extracellular domain with four potential N‑linked glycosylation sites, a transmembrane sequence, and a very short (approximately 6 aa) cytoplasmic sequence (6). SECTM1 proteins from human and mouse show species‑specific binding of CD7 and co‑stimulation of T cells, including enhancement of CD3‑induced proliferation (2‑4).

• References:

1. Slentz-Kesler, K.A. et al. (1998) Genomics 47:327.

2. Lam, G.K. et al. (2005) J. Clin. Immunol. 25:41.

3. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.

4. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.

5. Huyton, T. et al. (2011) Biochim. Biophys. Acta 1810:1294.

6. Swiss-Prot Accession # Q921W8.

• Long Name:

  Secreted and Transmembrane 1A

• Entrez Gene IDs:

  209588 (Mouse); 287885 (Rat)

• Alternate Names:

  SECTM1A