• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED    ₅₀ for this effect is 2-10 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 30 ng/mL of rhIL-2.

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived

  Mouse Common gamma Chain (Ser25 - Ala263) Accession # Q3UPA9	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ser25

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  54.5 kDa (monomer)

• SDS-PAGE

  60 kDa, reducing conditions

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma  is also designated the common gamma chain ( gamma _c) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.

• References:

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.

2. Noguchi, M. et al. (1993) Science 262:1877.

• Long Name:

  IL-2 Receptor gamma

• Entrez Gene IDs:

  3561 (Human); 16186 (Mouse)

• Alternate Names:

  CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency