• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED    ₅₀ for this effect is 0.15 - 0.9 µg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Leu18-Ala716, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained, Leu18 predicted. Protein identity confirmed by MS analysis of tryptic fragments.

• Predicted Molecular Mass

  77 kDa (monomer)

• SDS-PAGE

  85-105 kDa, reducing conditions

Background: Protocadherin-12

Protocadherin-12 (PCDH12; also called VE-Cadherin-2) is an approximately 150 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins. These are type I transmembrane proteins that exhibit calcium-dependent but relatively weak homophilic adhesion (1-4). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1). Within the ECD, mouse Protocadherin-12 shares 95% and 82% amino acid sequence identity with rat and human Protocadherin-12, respectively. Protocadherin-12 is expressed in mouse endothelial, glycogen-rich trophoblast, and mesangial cells where it clusters at intercellular junctions (1, 2). It may be important during placental development, as placentas from Protocadherin-12-deficient mice display altered morphogenesis and transcription profiles (3). In humans, the ECD of Protocadherin-12 can be shed   via proteolytic cleavage (5). It circulates at increased levels in sera from pregnant women exhibiting pre-eclampsia compared to women with nonpathological gestations (5).

• References:

1. Telo’, P. et al. (1998) J. Biol. Chem. 273:17565.

2. Rampon, C. et al. (2005) Exp. Cell Res. 302:48.

3. Rampon, C. et al. (2008) Physiol. Genomics 34:193.

4. Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.

5. Bouillot, S. et al. (2011) J. Biol. Chem. 286:15195.

• Entrez Gene IDs:

  51294 (Human); 53601 (Mouse)

• Alternate Names:

  PCDH12; protocadherin 12; Protocadherin12; Protocadherin-12; Vascular cadherin-2; vascular endothelial cadherin 2; Vascular endothelial cadherin-2; VECAD2; VE-cad-2; VE-cadherin 2; VE-cadherin-2; VE-cadherin-2protocadherin-12