• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Mouse SECTM1 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse CD7 that produces 50% optimal binding response is approximately 15‑75 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived Gln24-Pro150, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln24 predicted

• Predicted Molecular Mass

  15 kDa

• SDS-PAGE

  20-35 kDa, reducing conditions

Background: CD7

CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily (1). Mature mouse CD7 consists of a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 39 aa cytoplasmic domain (2). Within the ECD, mouse CD7 shares 43% and 81% aa sequence identity with human and rat CD7, respectively. CD7 is expressed on T cells (2, 3), NK cells (4), myeloid progenitor cells (5), and CD19  ⁺ B progenitor cells (6). Among CD8  ⁺ T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells (7). CD7 associates   in cis with CD3 and CD45 and   in trans with SECTM1 (8, 9). Ligation of CD7 with antibodies or SECTM1 co-stimulates the activation and proliferation of CD4  ⁺ and CD8  ⁺ T cells (10, 11) and NK cells (4, 9). It also enhances integrin-mediated adhesion of these cells to Fibronectin, ICAM-1, and VCAM-1 (3, 12). CD7 additionally mediates the Galectin-1 induced apoptosis of T cells (13) and facilitates the fusion of HIV-1 with target cells (14).

• References:

1. Sutherland, D.R. et al. (1984) J. Immunol. 133:327.

2. Yoshikawa, K. et al. (1993) Immunogenetics 37:114.

3. Shimizu, Y. et al. (1992) J. Exp. Med. 175:577.

4. Rabinowich, H. et al. (1994) J. Immunol. 152:517.

5. Chabannon, C. et al. (1992) J. Immunol. 149:2110.

6. Grumayer, E.R. et al. (1991) Blood 77:64.

7. Aandahl, E.M. et al. (2003) J. Immunol. 170:2349.

8. Lazarovits, A.I. et al. (1994) J. Immunol. 153:3956.

9. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.

10. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.

11. Carrera, A.C. et al. (1988) J. Immunol. 141:1919.

12. Rabinowich, H. et al. (1994) J. Immunol. 153:3504.

13. Pace, K.E. et al. (2000) J. Immunol. 165:2331.

14. Sato, A.I. et al. (1994) J. Immunol. 152:5142.

• Entrez Gene IDs:

  924 (Human); 12516 (Mouse); 303747 (Rat)

• Alternate Names:

  CD7 antigen (p41); CD7 antigen; CD7 molecule; CD7; GP40T-cell surface antigen Leu-9; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; Tp40; TP41; TP41p41 protein