• Purity

  >90%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED    ₅₀ for this effect is 0.7-3.5 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Gln25-Phe440, with an N-terminal HA Tag

• Accession #

• N-terminal Sequence    
  Analysis

  Tyr 

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  47 kDa

• SDS-PAGE

  53-63 kDa, reducing conditions

Background: Fibulin 7

Fibulin 7, also known as TM14, is an approximately 46 kDa member of the fibulin family of proteins (1, 2). Structurally, Fibulin 7 contains an N-terminal Sushi domain, 3 central tandem EGF modules, and a C-terminal domain homologous to the fibulin module (2). Mouse Fibulin 7 mRNA is highly expressed in teeth, with weaker expression in brain, kidneys, muscles, and bones (2). Mouse Fibulin 7 shares 86% amino acid sequence identity with human Fibulin 7. Fibulin 7 interacts with several extracellular molecules, including Fibronectin, DSPP, and Fibulin 1 (2). A C-terminal fragment of Fibulin 7 was able to bind Human Umbilical Vein Endothelial Cells (HUVECs) and inhibit HUVEC tube formation and vessel sprouting in aortic ring assays, suggesting that Fibulin 7 may have anti-angiogenic activity (3). Fibulin 7 may be important for proper development, since it is included in a chromosomal deletion that is associated with severe cardiac defects and craniofacial anomalies (4).

• References:

1. Gallagher, W.M. et al. (2005) Trends Mol. Med. 11:336.

2. de Vega, S. et al. (2007) J. Biol. Chem. 282:30878.

3. de Vega, S. et al. (2014) Arch. Biochem. Biophys. 545:148.

4. Russell, M.W. et al. (2014) Hum. Mol. Genet. 23:4272.

• Entrez Gene IDs:

  129804 (Human); 70370 (Mouse); 296145 (Rat)

• Alternate Names:

  FBLN7; FIBL-7; Fibulin 7; TM14