• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA.

  When Mannan is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Rat MMR/CD206 that produces 50% optimal binding response is 0.1-0.5 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Leu26-Ala1395, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Starts at Leu26

• Predicted Molecular Mass

  156.4 kDa

• SDS-PAGE

  150-180 kDa, reducing conditions

Background: MMR/CD206

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C-type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor-associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1463 amino acid (aa) rat CD206 precursor contains a signal sequence (26 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II (FNII) domain, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The rat CD206 ECD shares 96% aa sequence identity with mouse CD206, and 84‑85% with human, equine, porcine and canine CD206. The cysteine‑rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewis  ^a and ‑Lewis  ^x (1, 7, 9). The FNII domain mediates Ca2  ⁺‑independent binding of collagens (2, 10). The CTLDs participate in Ca2  ⁺‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin‑coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

• References:

1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.

2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.

3. Harris, N. et al. (1992) Blood 80:2363.

4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.

5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.

6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.

7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.

8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.

9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.

10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.

11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.

12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

• Long Name:

  Macrophage Mannose Receptor

• Entrez Gene IDs:

  4360 (Human); 17533 (Mouse); 291327 (Rat)

• Alternate Names:

  CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1