• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 Ligand and anti-CD3. The ED    ₅₀ for this effect is 0.4-1.6 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Rat CD27/TNFRSF7 (Thr21-Arg183) Accession # NP_001019506	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Thr21

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  45.1 kDa (monomer)

• SDS-PAGE

  60-66 kDa, reducing conditions

Background: CD27/TNFRSF7

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival (1). Mature rat CD27 consists of a 163 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 47 aa cytoplasmic domain. Within the ECD, rat CD27 shares 66% and 85% aa sequence identity with human and mouse CD27, respectively. CD27 is expressed as a disulfide‑linked homodimer that carries N‑linked and O‑linked glycosylation (2, 3). Proteolytic cleavage of CD27 results in the shedding of a 28‑32 kDa fragment of the ECD (3). CD27 is weakly expressed on naïve T cells and NK cells and is up‑regulated upon cell activation (3, 4). It is also up‑regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells (5, 6). CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells (1, 7, 8). This interaction contributes to the activation and survival of CD4  ⁺ helper T cells (7‑10), CD8  ⁺ effector T cells (11, 12), memory T cells (9), and NK cells (4, 13). Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses (5, 14).

• References:

1. Nolte, M.A. et al. (2009) Immunol. Rev. 229:216.

2. van Lier, R.A. et al. (1987) J. Immunol. 139:1589.

3. Loenen, W.A. et al. (1992) Eur. J. Immunol. 22:447.

4. Takeda, K. et al. (2000) J. Immunol. 164:1741.

5. Xiao, Y. et al. (2004) J. Immunol. 172:7432.

6. Jung, J. et al. (2000) Eur. J. Immunol. 30:2437.

7. Goodwin, R.G. et al. (1993) Cell 73:447.

8. Bowman, M.R. et al. (1994) J. Immunol. 152:1756.

9. Hendriks, J. et al. (2000) Nat. Immunol. 1:433.

10. van Oosterwijk, M.F. et al. (2007) Int. Immunol. 19:713.

11. Rowley, T.F. and A. Al Shamkhani (2004) J. Immunol. 172:6039.

12. Hendriks, J. et al. (2003) J. Exp. Med. 198:1369.

13. Kelly, J.M. et al. (2002) Nat. Immunol. 3:83.

14. Raman, V.S. et al. (2003) J. Immunol. 171:5876.

• Entrez Gene IDs:

  939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

• Alternate Names:

  CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7