• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Rat CLEC10A/CD301 is immobilized at 2.5 µg/mL (100 µL/well), the concentration of biotinylated Recombinant Viral EBOV GP that produces 50% of the optimal binding response is approximately 1-6 μg/mL

• Source

  Chinese Hamster Ovary cell line, CHO-derived Gln59-Ser306, with an N-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  His    
   

• Predicted Molecular Mass

  29 kDa

• SDS-PAGE

  34-42 kDa, reducing conditions

Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein in the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Rat CLEC10A consists of a 37 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 248 aa extracellular domain (ECD) with one carbohydrate recognition domain and a neck region (2). Within the ECD, rat CLEC10A shares 56% and 79% aa sequence identity with human CLEC10A and mouse CLEC10A/MGL1, respectively. CLEC10A is expressed on immature myleloid dendritic cells, Langerhans cells, and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-8). It is up-regulated on pro-inflammatory monocytes in obesity and promotes their trafficking to adipose tissue (9). CLEC10A selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates including the Tn carcinoma antigen (3, 4, 10-12). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (13). CLEC10A expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) on T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (14).

• References:

1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.

2. Ii, M. et al. (1990) J. Biol. Chem. 265:11295.

3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686.

4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.

5. van Vliet, S.J. et al. (2006) Immunobiology 211:577.

6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.

7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.

8. Dupasquier, M. et al. (2006) J. Leukoc. Biol. 80:838.

9. Westcott, D.J. et al. (2009) J. Exp. Med. 206:3143.

10. Suzuki, N. et al. (1996) J. Immunol. 156:128.

11. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.

12. Higashi, N. et al. (2002) Int. Immunol. 14:545.

13. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.

14. Takada, A. et al. (2004) J. Virol. 78:2943.

• Long Name:

  Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1

• Entrez Gene IDs:

  10462 (Human)

• Alternate Names:

  CD301 antigen; CD301; CD301C-type lectin domain family 10 member A; CLEC10A; CLECSF13; CLECSF14; CLECSF14macrophage C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived); C-type lectin domain family 10, member A; C-type lectin superfamily member 14; HML2; HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived); macrophage lectin 2 (calcium dependent); Macrophage lectin 2; MGL