• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized at 3 μg/mL on a nitrocellulose-coated microplate.

• Source

  Mouse myeloma cell line, NS0-derived

  Rat Nogo-A (Met1-Val172) Accession # Q9JK11	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Met1

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  45 kDa (monomer)

• SDS-PAGE

  66-76 kDa, reducing conditions

Background: Nogo-A

Nogo, so named as a “No-Go” for neurite outgrowth, is a member of the reticulon family of transmembrane proteins, and is also called reticulon 4 (gene name RTN4) (1-4). Reticulons lack N-terminal signal sequences, share a conserved ~200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER‑retention motif, and show a punctate intracellular distribution within the endoplasmic reticulum (ER) that is reminescent of a reticulum (1-3). The N-terminus of intracellular (ER) Nogo-A appears to face the cytoplasm (1-3). However, minor amounts of Nogo-A and Nogo-B are found in the plasma membrane with extracellular N-termini (4-6). Full length rat Nogo-A is a 1163 aa protein with a long (~989 aa) N-terminus that includes bioactive regions (aa 59-172 and 544-725), a transmembrane segment, a connecting loop that contains the bioactive Nogo-66 region, a second transmembrane segment, and a short C-terminus (3). The four Nogo isoforms share the Nogo66 segment, Nogo-A and Nogo-B share aa 1-172, and only Nogo-A contains aa 544-725 (1-3). Rat Nogo-A shares 78% and 91% aa sequence identity with human and mouse Nogo-A, respectively. Rat and human Nogo-A/B also share 78% aa sequence identity within aa 1-172 and 98% within the Nogo-66 loop region. Nogo-A is mainly expressed in oligodendrocytes of the central nervous system, but is also reported in fibroblasts, dorsal root ganglion neurons, macrophages and myoblasts (1-8). Nogo-B is mainly expressed in vascular endothelium and smooth muscle throughout the body (1, 4, 6, 9). The Nogo66 region binds the GPI-linked Nogo receptor/p75 complex on axons, inducing growth cone collapse (5, 7, 10, 11). Either aa 59-172 or 544-725 segments can block neurite outgrowth and fibroblast spreading (5, 6). Nogo-A/B aa 1-172 is also reported to regulate vascular remodeling through binding the Nogo-B receptor (NgBR/NUS1) on vascular cells, and to inhibit neuronal differentiation and promote glial formation from neural progenitors (4, 5, 8, 9, 12).

• References:

1. Oertle, T. and M.E. Schwab (2003) Trends Cell Biol. 13:187.

2. GrandPre, T. et al. (2000) Nature 403:439.

3. Chen, M.S. et al. (2000) Nature 403:434.

4. Acevedo, L. et al. (2004) Nat. Med. 10:382.

5. Oertle, T. et al. (2003) J. Neurosci. 23:5393.

6. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.

7. Wang, X. et al. (2002) J. Neurosci. 22:5505.

8. Schwab, M.E. (2010) Nat. Rev. Neurosci. 11:799.

9. Miao, R.Q. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10997.

10. Fournier, A.E. et al. (2001) Nature 409:341.

11. Wang, K.C. et al. (2002) Nature 420:74.

12. Guo, Y. et al. (2009) Neurosci. Lett. 458:132.

• Long Name:

  Reticulon 4A

• Entrez Gene IDs:

  57142 (Human); 68585 (Mouse); 83765 (Rat)

• Alternate Names:

  NI220; NogoA; Nogo-A; RTN4; RTN4A