详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED 50 for this effect is 0.2-0.6 ng/mL.
- SourceE. coli-derived Ala21-Thr154, with an N-terminal Met
- Accession #
- N-terminal Sequence
AnalysisMet - Predicted Molecular Mass15.3 kDa
| 652-P2/CF | | 652-P2 |
| Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. | Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. | |
| Reconstitution Reconstitute at 50 μg/mL in sterile PBS. | Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that in addition to its T cell growth factor activity, IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. At the amino acid sequence level, there is approximately 72% similarity between mature porcine and human IL-2.
The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor of IL-2 is composed of three distinct polypeptide chains, the IL-2 receptor alpha, beta and gamma subunits. The intermediate-affinity IL-2 receptor complex, which lacks the alpha subunit, but contains both the beta and gamma subunits, is also capable of transducing the IL-2 signal. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma chain of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9.
- References:
- Taniguchi, T. and Y. Minami (1993) Cell 73:5.
- Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
- Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
- Long Name:Interleukin 2
- Entrez Gene IDs:3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
- Alternate Names:aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
400-6226-992