详细说明
- Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit LPS-induced IL‑1 beta secretion by human peripheral blood lymphocytes (PBL). The ED 50 for this effect is 0.1-0.6 ng/mL.
- SourceE. coli-derived Ala26-Lys176
- Accession #
- N-terminal Sequence
AnalysisAla26 - Structure / FormNoncovalently-linked homodimer
- Predicted Molecular Mass17.6 kDa
| 117-VL/CF | | 117-VL |
| Formulation Lyophilized from a 0.2 μm filtered solution in NaH 2PO 4 and NaCl. | Formulation Lyophilized from a 0.2 μm filtered solution in NaH 2PO 4 and NaCl with BSA as a carrier protein. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen‑stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).
- References:
- Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
- Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
- Spencer, J.V. et al. (2002) J. Virol. 76:1285.
- Long Name:Interleukin 10
- Entrez Gene IDs:3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
- Alternate Names:CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
400-6226-992