详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.01 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED 50 for this effect is 3-15 ng/mL.
- SourceE. coli-derived Ala29-Asn101
- Accession #
- N-terminal Sequence
AnalysisAla29 - Predicted Molecular Mass8.0 kDa
| 1084-M2/CF | | 1084-M2 |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Macrophage inflammatory protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The protein is produced by a variety of cell types, including intestinal epithelium, macrophages, astrocytes and fibroblasts. Homologs of the mouse MIP-2 have been identified in human, rat and cotton rat. MIP-2 is an ELR-containing member of the alpha (CXC) subfamily of chemokines. The cotton rat MIP-2 gene encodes a 101 amino acid (aa) residue precursor protein with a 28 aa putative signal peptide that is cleaved to generate the 73 aa mature protein. Mature cotton rat MIP-2 has two intrachain disulfide bonds and no potential glycosylation sites. It shares approximately 79% and 85% aa sequence identity with rat CINC-3 and mouse MIP-2, respectively. MIP-2 is a potent neutrophil attractant and activator. MIP-2 binds and activates the chemokine receptor CXCR2. The recombinant cotton rat MIP-2 has been shown to bind and activate the human CXCR2 (1 - 5).
- References:
- Yoshie, O. et al. (2001) Adv. Immunol. 78:57.
- Nakagawa, H. et al. (1994) Biochem. J. 301:545.
- Telkamp-Olson, P. et al. (1990) J. Exp. Med. 172:911.
- Lee, J. et al. (1995) J. Immunol. 155:2158.
- Feng, L. et al. (1995) J. Clin. Invest. 95:1009.
- Entrez Gene IDs:2920 (Human); 20310 (Mouse); 114105 (Rat)
- Alternate Names:chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2
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