详细说明
- Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED 50 for this effect is 0.4-2 ng/mL.
- SourceE. coli-derived Ala28- Lys100
- Accession #
- N-terminal Sequence
AnalysisAla28 - Predicted Molecular Mass7.9 kDa
| 1114-GR/CF | | 1114-GR |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Cotton rat growth-regulated protein (GRO) is a member of the ELR+ CXC subfamily of chemokines (1). The cotton rat GRO cDNA encodes a 100 amino acid (aa) residue precursor protein with a 27 aa putative signal peptide and a 73 aa mature protein (2). Among the three human GRO proteins, mature cotton rat GRO is most closely related to GRO alpha /CXCL1, sharing approximately 77% aa sequence identity (3). It also shares 75% and 70% aa sequence identity with mature human GRO beta /CXCL2 and GRO gamma /CXCL3, respectively (4). Compared with rat CXC chemokines, mature cotton rat shows 92%, 76%, and 76% aa sequence homology with rat KC/GRO/CINC-1, CINC-2 and CINC-3, respectively (5). Mature cotton rat GRO also shares 74% sequence homology with mature cotton rat MIP-2 (6). Similarly to GRO proteins from other species, cotton rat GRO is expected to be a potent neutrophil chemoattractant and activator. Cotton rat GRO has been shown to bind and activate both mouse chemokine receptors CXCR1 and CXCR2 (7). Similarly to human GRO alpha, the cotton rat GRO concentration required to activate CXCR1 is at least an order of magnitude higher than that required to activate CXCR2 (7, 8). Based on studies on rodents, cotton rat GRO is predicted to be produced by a wide variety of cell types including macrophages, endothelial cells, fibroblasts, astrocytes, neutrophils, keratinocytes and intestinal epithelium (1, 9).
- References:
- Rollins, B.J. (1997) Blood 90:909.
- Blanco, J.C. et al. (2001) GenBank Accession #: AAL16934.
- Richmond, A. et al. (1988) EMBO J. 7:2025.
- Haskill, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7732.
- Nakagawa, H. et al. (1994) Biochem. J. 301:545.
- Blanco, J.C. et al. (2001) GenBank Accession #: AAL26705.
- R&D Systems, unpublished observations.
- Schumacher, C. et al. (1992) Proc. Natl. Acad. Sci. USA 89:10542.
- Zlotnik, A.O. (2000) Immunity 12:121.
- Alternate Names:CXCL1/2/3
400-6226-992