详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rcNetrin-2 with a linear range of 6-400 ng/mL.
- SourceMouse myeloma cell line, NS0-derived Ala16-Pro581, with a C-terminal 6-His tag & Ala16-Pro581, with an N-terminal Met and C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisAla16 & Met
- Predicted Molecular Mass64 kDa
- SDS-PAGE80 kDa, reducing conditions
| 127-N2/CF | | 127-N2 |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Netrins are laminin-like axon guidance molecules that are conserved among C. elegans, Drosophila, and vertebrates. The chicken Netrin-2 cDNA encodes a precursor protein that has structural similarity to the N-terminus of B2 or the gamma -chain of laminin. The mature Netrin-2 protein has 581 amino acid (aa) which contains a 245 aa domain VI, a 169 aa domain V with three cysteine/glycine rich EGF repeats and a unique 152 aa domain C that enriches in basic aa residues and contains an RGD motif. Chicken Netrin-2 shares 72% aa identity with chicken Netrin-1, 51% aa identity with C. elegans UNC-6 protein and 57% aa identity with human NTN2L (Netrin‑2‑like). Although only two chicken netrins are known, the number of known mammalian netrins is growing. Mouse Netrin-3 and Netrin-4 were recently reported. Netrin-2 was expressed in the chick brain and spinal cord from E6 through to the adult. In addition, expression of Netrin-2 was observed at various stages in several mesodermally and endodermally derived tissues, including lung, gut, ovary, testes and spleen. Netrins act to both attract and repel the growing axons of a broad range of neuronal cell types during development. These actions are mediated by specific receptor complexes containing either the DCC (colorectal carcinoma) or neogenin, in the case of attractant, or UNC-5-related proteins, in the case of repellent.
- References:
- Serafini, T. et al. (1994) Cell 78:409.
- Kennedy, T.E. et al. (1994) Cell 78:425.
- Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
- Entrez Gene IDs:4917 (Human); 396387 (Chicken)
- Alternate Names:Netrin2; Netrin-2; NTN2L; NTN3
400-6226-992