详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED 50 for this effect is 1-5 ng/mL.
- SourceSpodoptera frugiperda, Sf 21 (baculovirus)-derived Met1-Glu296, Leu117-Glu296 & Ala162-Glu296, each with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisMet1, Leu117 & Ala162
- Predicted Molecular Mass33 kDa
- SDS-PAGE25-45 kDa, reducing conditions
| 378-SM/CF | | 378-SM |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
The heregulin (also known as neuregulin) family of cytokines is comprised of multiple secreted or membrane-bound isoforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. All heregulin family members share an epidermal growth factor (EGF)-like domain ( alpha - or beta -variant) that interacts with the erbB family of tyrosine kinase receptors. NRG1 Isoform SMDF is a heregulin isoform containing a C-terminal EGF-like domain ( beta -variant) and a unique N-terminal sequence that lacks an Ig-like domain which is present in all other known heregulins. NRG1 Isoform SMDF also lacks a transmembrane domain and the cytoplasmic tail. NRG1 Isoform SMDF expression has been found to be restricted to the nervous system. It is likely that NRG1 Isoform SMDF may play an important role in neural-specific functions.
- References:
- Yarden, Y. and D. Wen (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
- Meyer, D. et al. (1997) Development 124:3575.
- Entrez Gene IDs:3084 (Human); 211323 (Mouse); 112400 (Rat)
- Alternate Names:Neuregulin1 Isoform SMDF; Neuregulin-1 Isoform SMDF; SMDF
400-6226-992