详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human XCR1. The ED 50 for this effecy is 10-60 ng/mL.
- SourceHuman embryonic kidney cell, HEK293-derived Val22-Gly114
- Accession #
- N-terminal Sequence
AnalysisVal22 - Predicted Molecular Mass10 kDa
- SDS-PAGE19-22 kDa, reducing conditions
| 8418-X2/CF | | 8418-X2 |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
| Reconstitution Reconstitute at 250 μg/mL in sterile PBS. | Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
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| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Bioactivity | Recombinant Human XCL2/SCM-1 beta (Catalog # 8418-X2/CF) chemoattracts BaF3 mouse pro B cells transfected with human XCR1. The ED50 for this effect is 10-60 ng/mL. |
Human XCL2 (also known as Lymphotactin-2, SCYC2, and SCM-1 beta ) is a 10 kDa glycosylated protein in the C chemokine family (1-3). XCL1/Lymphotactin is a closely related chemokine that differs from XCL2 by only two amino acids (aa). Human XCL2 encodes a 114 amino acid aa precursor protein with a 21 aa predicted signal peptide. Members of the C chemokine family lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines. They also have an extended carboxy terminus (4). XCL1/Lymphotactin which is expressed in activated T cells and NK cells, while certain stimulatory conditions have been shown to induce XCL2 expression in macrophages (5-7). XCL2 and XCL1/Lymphotactin bind and activate the G protein-coupled receptor, XCR1. XCR1 is expressed on dendritic cells, T lymphocytes, and B lymphocytes (8-11). Binding of XCL2 to XCR1 initiates chemotaxic cell migration in dendritic cells as well as in T and B lymphocytes. XCR1 is also expressed on ovarian carcinoma cells and may contribute to cancer cell migration and proliferation in response to XCL2 or XCL1/Lymphotactin (12).
- References:
- Yoshida, T. et al. (1996) FEBS Lett. 395:82.
- Kelner, G.S. et al. (1994) Science 266:1395.
- Muller, S. et al. (1995) Eur. J. Immunol. 25:1744.
- Yoshida, T. et al. (1995) FEBS Lett. 360:155.
- Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
- Luzi, G. et al. (2007) Clin. Immunol. 125:127.
- Cao, W. et al. (2008) Curr. Microbiol. 57:189.
- Kroczek, R.A. and V. Henn (2012) Front. Immunol. 3:14.
- Huang, H. et al. (2001) Biochem. Biophys. Res. Commun. 281:378.
- Pigard, N. et al. (2009) J. Neuroimmunol. 209:114.
- Yamazaki, C. et al. (2010) Biochem. Biophys. Res. Commun. 397:756.
- Kim, M. et al. (2012) Mol. Cancer. Res. 10:1419.
- Long Name:XCL 2/SCM-1 beta
- Entrez Gene IDs:6846 (Human)
- Alternate Names:C motif chemokine 2; chemokine (C motif) ligand 2; cytokine SCM-1 beta; SCM1 beta; SCM-1 beta; SCM1B; SCM-1b; SCYC2; SCYC2XC chemokine ligand 2; small inducible cytokine subfamily C, member 2; XCL2
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