详细说明
- Purity>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to enhance LPS-stimulated IL-8 secretion by THP‑1 human acute monocytic leukemia cells. The ED 50 for this effect is 0.25‑2.5 ng/mL.
- SourceMouse myeloma cell line, NS0-derived Ala26-Val481
- Accession #
- N-terminal Sequence
AnalysisAla26 - Predicted Molecular Mass51 kDa
- SDS-PAGE55-60 kDa, reducing conditions
| 870-LP | | 870-LP/CF |
| Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA. | |
| Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Lipopolysaccharide-binding protein (LBP), a 58 kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins which includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipid A portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyze the binding of LPS monomers to CD14, and promote LPS-induced immune response. LBP is present at low concentrations in normal human serum and may increase up to 30-fold during the acute phase response. Studies indicate that LBP also acts catalytically in the transfer of LPS to HDL, thus accelerating LPS detoxification. In addition, LBP and soluble CD14 can also function in phospholipid transport.
- References:
- Hailman, E. et al. (1994) J. Exp. Med. 179:269.
- Jack, R.S. et al. (1997) Nature 389:742.
- Schumann, R.R. et al. (2000) Chem. Immunol. 74:42.
- Long Name:Lipopolysaccharide-binding Protein
- Entrez Gene IDs:3929 (Human); 16803 (Mouse)
- Alternate Names:LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233
400-6226-992