• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to promote the expansion of E16 rat liver mononuclear cells     in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # ), Recombinant Mouse Thrombopoietin/Tpo (Catalog # ), and Recombinant Mouse Flt‑3 Ligand (Catalog # ). The ED    ₅₀ for this effect is 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # ).

• Source

  Chinese Hamster Ovary cell line, CHO-derived Met1-Pro346, with a C-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln27 predicted

• Predicted Molecular Mass

  38.6 kDa

• SDS-PAGE

  49-55 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Angiopoietin-like Protein 7/ANGPTL7

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1 - 3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45 ‑ 50 kDa monomer that forms disulfide-linked homotrimers and tetramers   via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5 - 7). Overexpression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When overexpressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells (9).

• References:

1. Oike, Y. et al. (2009) Circ. J. 73:2192.

2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.

3. Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782.

4. Peek, R. et al. (2002) J. Biol. Chem. 277:686.

5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438.

6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58.

7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.

8. Comes, N. et al. (2010) Genes Cells 16:243.

9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

• Entrez Gene IDs:

  10218 (Human); 654812 (Mouse)

• Alternate Names:

  angiopoietin-like 7; Angiopoietin-like factor; Angiopoietin-like Protein 7; angiopoietin-related protein 7; ANGPTL7; AngX; CDT6; CDT6angiopoietin-like factor (CDT6); Cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2