• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind rrMAG/Fc Chimera in a functional ELISA.

• Source

  Mouse myeloma cell line, NS0-derived

  Human Nogo Receptor (Cys27 - Ser 447) Accession # Q9BZR6	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Cys27

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  71.8 kDa (monomer)

• SDS-PAGE

  95-105 kDa, reducing conditions

Background: Nogo Receptor/NgR

Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid residues (aa) precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75^NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75^NTR. The activated p75^NTR then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).

• References:

1. Fournier, A.E. et al. (2001) Nature 409:341.

2. GrandPre, T. et al. (2002) Nature 417:547.

3. Wang, K.C. et al. (2002) Nature 420:74.

4. Barton, W.A. et al. (2003) EMBO Journal 22:3291.

5. Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.

6. Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.

• Long Name:

  Nogo-66 Receptor/Reticulon 4 Receptor

• Entrez Gene IDs:

  65078 (Human); 65079 (Mouse)

• Alternate Names:

  NgR; NgR1; Nogo R; Nogo receptor; Nogo-66 receptor; NogoR; NOGORUNQ330/PRO526; reticulon 4 receptor; reticulon-4 receptor; RTN4R