详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED 50 for this effect is 0.5-2 ng/mL.
- SourceE. coli-derived Gly42-Ala116
- Accession #
- N-terminal Sequence
AnalysisGly42 - Predicted Molecular Mass8.3 kDa
3210-C | | 3210-C/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Bioactivity | Recombinant MouseCCL6/C10 (Catalog # 3210-C) chemoattracts BaF3 mouse pro B cells transfected with human CCR1. The ED50 for this effect is 0.5-2 ng/mL. |
CCL6, also known as C10 and SCYA6, is an 11 kDa member of the MIP-I family of beta -chemokines. CCL6, along with CCL9, CCL15, and CCL23, belongs to the N6 subfamily of proteins that have an N-terminal extension relative to other beta -chemokines (1 - 4). The mouse CCL6 cDNA encodes a 116 amino acid (aa) precursor that includes a 21 aa signal sequence (5). Removal of an additional 20 aa at the N-terminal results in an 8 kDa protein (aa 42 - 116) and the transition from a weak CCR1 agonist to a dramatically more potent and efficacious macrophage chemoattractant (3, 6). The N-terminal region of CCL6 and other N6 subfamily chemokines is cleaved following incubation with synovial fluid from inflamed joints as well as with inflammation-associated proteases such as chymase, cathepsin G, and elastase (3). CCL6 is upregulated in activated macrophages at sites of inflammation (5, 7 - 10) and functions as a chemoattractant for additional macrophage influx (8, 9). The inflammatory cytokine IL-13 stimulates CCL6 production which then induces the release of CCL2, CCL3, and proteases involved in tissue repair (10). CCL6 expression is upregulated by the oncoprotein L-Myc but repressed by c-Myc. CCL6 induced by L-Myc promotes cellular transformation and the development of a tumorigenic phenotype (11). N-terminally truncated mouse CCL6 shares 77% and 64% aa sequence identity with rat CCL6 and mouse CCL9, respectively. A human orthologue for mouse CCL6 has not been identified. Conversely, CCL15 and CCL23 and other members of the N6 subfamily have been described in human but not in mouse.
- References:
- Maurer, M. and E. von Stebut (2004) Int. J. Biochem. Cell Biol. 36:1882.
- Trakatelli, C. et al. (2005) Int. J. Biol. Markers 20:197.
- Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
- Berger, M.S. et al. (1993) DNA Cell Biol. 12:839.
- Orlofsky, A. et al. (1991) Cell Regul. 2:403.
- Berger, M.S. et al. (1996) Cytokine 8:439.
- Orlofsky, A. et al. (1994) J. Immunol. 152:5084.
- Kaesler, S. et al. (2002) Cytokine 3:157.
- LaFleur, A.M. et al. (2004) Mediators Inflamm. 13:349.
- Ma, B. et al. (2004) J. Immunol. 172:1872.
- Yi, F. et al. (2003) Cancer Res. 63:2923.
- Entrez Gene IDs:20305 (Mouse); 287910 (Rat)
- Alternate Names:C10; CCL6; MRP-1; SCYA6