详细说明
- Purity>95%, by SDS-PAGE with silver staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human TSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The ED 50 for this effect is 0.015-0.15 ng/mL.
- SourceChinese Hamster Ovary cell line, CHO-derived
Mouse TSH beta
(Phe21-Val138)
Accession # P12656(GGGS)3 Mouse TSH alpha
(Leu25-Ser120)
Accession # P01216N-terminus C-terminus - N-terminal Sequence
AnalysisPhe21 (mTSH beta ) - Predicted Molecular Mass25 kDa
- SDS-PAGE35-41 kDa, reducing conditions
8885-TH/CF | | 8885-TH |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
Reconstitution Reconstitute at 100 μg/mL in PBS. | Reconstitution Reconstitute at 100 μg/mL in PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
half-lives exist due to differences in the post-translational glycosylation and sialylation modifications of its subunits (1, 4). TSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Thyrotropin-Releasing Hormone (TRH) from the hypothalamus. TSH travels to the thyroid gland and binds to the TSH Receptor to stimulate production of Thyroxine (T4), which is converted in peripheral tissues to Triiodothyronine (T3), a more biologically potent form of the thyroid hormone (1, 4, 5). TSH secretion is also controlled by plasma T3 and T4 via a negative feedback mechanism and by the neurotransmitters dopamine and Somatostatin (4, 5). Serum TSH levels are often assessed to evaluate thyroid function (4, 5). TSH has also been shown to be produced by bone marrow and is believed to have a direct effect on skeletal homeostasis by promoting bone strength and quality (6-9). Additionally, serum TSH levels have been shown to be decreased in the elderly (10).
- References:
- Szkudlinshi, M.W. et al. (2002) Physiol. Rev. 82:473.
- Farid, N.R. and M.W. Szkudlinski (2004) Endocrinology 145:4048.
- Cahoreau, C. et al. (2015) Front. Endocrinol. (Lausanne) 6:26.
- Roelfsema, F. and J.D. Veldhuis (2013) Endocr. Rev. 34:619.
- Winter, W.E. and M.R. Signorino (2001) Ann. Clin. Lab. Sci. 31:221.
- Klein, J.R. and H.C. Wang (2004) J. Exp. Biol. 207:55.
- Abe, E. et al. (2003) Cell 115:151.
- Sun, L. et al. (2006) Ann. N.Y. Acad. Sci. 1068:309.
- Sendak, R.A. et al. (2007) Int. Orthop. 31:753.
- Peeters, R.P. (2008) Hormones 7:28.
- Long Name:Thyroid Stimulating Hormone
- Alternate Names:Thyrotropin; TSH alpha/beta Heterodimer