详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Mouse R‑Spondin 3 (Catalog # ) is coated at 0.25 μg/mL, Recombinant Mouse RNF43 Fc Chimera binds with an ED 50 = 0.8-4.8 ng/mL.
- SourceMouse myeloma cell line, NS0-derived
Mouse RNF43
(Ala34-Tyr197)
Accession # Q5NCP0IEGRMDP Mouse IgG2a
(Glu98-Lys330)N-terminus C-terminus - N-terminal Sequence
AnalysisAla34 & Val36
- Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass45 kDa
- SDS-PAGE53-63 kDa, reducing conditions
8890-RN | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- References:
- Yagyu, R. et al. (2004) Int. J. Oncol. 25:1343.
- de Lau, W. et al. (2014) Genes Dev. 28:305.
- Koo, B.K. et al. (2012) Nature 488:665.
- Hao, H.X. et al. (2012) Nature 485:195.
- Tsukiyama, T. et al. (2015) Mol. Cell. Biol. 35:2007.
- Jiang, X. et al. (2015) Mol. Cell 58:522.
- Xing, C. et al. (2013) Mol. Cancer Ther. 12:94.
- Shinada, K. et al. (2011) Biochem. Biophys. Res. Commun. 404:143.
- Giannakis, M. et al. (2014) Nat. Genet. 46:1264.
- Xi, S. et al. (2015) Int. J. Clin. Exp. Pathol. 8:490.
- Uchida, N. et al. (2004) Clin. Cancer Res. 10:8577.
- Long Name:Ring Finger Protein 43
- Entrez Gene IDs:54894 (Human); 207742 (Mouse); 303412 (Rat)
- Alternate Names:DKFZp781H02126; DKFZp781H0392; FLJ20315; FLJ77466; FLJ99338; MGC125630; ring finger protein 43; RNF124; RNF43; URCC