• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED    ₅₀ for this effect is 0.3-1.5 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Leu25-Leu819, with an N-Terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  His

• Predicted Molecular Mass

  90 kDa

• SDS-PAGE

  105-130 kDa, reducing conditions

Background: NELL2

NELL2 is an approximately 140 kDa secreted glycoprotein that exhibits several actions during nervous system development. NELL2 associates into a homotrimer and contains one Laminin G domain, one vWF-C domain, and six EGF-like domains, followed by one more vWF-C domain (1). Mature mouse NELL2 shares 94% and 97% aa sequence identity with human and rat NELL2, respectively. Alternative splicing generates a cytosolic isoform that associates with PKC beta 1 and can inhibit PKC signaling (2). NELL2 is widely expressed in neurons of the central and peripheral nervous systems, particularly in the hippocampus, hypothalamus, cerebral cortex, and cerebellar cortex (3-5). It is selectively expressed in VGAT1+ or VGAT2+ glutamatergic neurons (4, 6). During development, NELL2 is expressed in select neural crest cells, retinal ganglion cells, and hypaxial muscle precursor cells of the dermomyotome (7). It promotes the differentiation and migration of both motor and sensory neurons (8), enhances neuronal survival (9), and promotes neuronal aggregation by inducing N-Cadherin up-regulation (10). NELL2 supports the release of GnRH from the hypothalamus which is important for the onset of female puberty (5, 6). NELL2 is additionally expressed in renal tubules but is down-regulated in renal cancer (11).

• References:

1. Watanabe, T.K. et al. (1996) Genomics 38:273.

2. Lee, D.Y. et al. (2014) Biochem. Biophys. Res. Commun. 454:459.

3. Oyasu, M. et al. (2000) Brain Res. Mol. Brain Res. 76:151.

4. Jeong, J.K. et al. (2008) Mol. Cells 26:186.

5. Zhou, S.S. and P. Li (2014) Genet. Mol. Res. 13:6672.

6. Ha, C.M. et al. (2008) Neuroendocrinology 88:199.

7. Nelson, B.R. et al. (2002) Mech. Dev. 119S:S11.

8. Nelson, B.R. et al. (2004) Dev. Biol. 270:322.

9. Aihara, K. et al. (2003) Mol. Brain Res. 116:86.

10. Kim, D.H. et al. (2014) PLoS One 9:e85898.

11. Nakamura, R. et al. (2015) Cancer Sci. 106:656.

• Long Name:

  NEL-like 2

• Entrez Gene IDs:

  4753 (Human); 54003 (Mouse); 81734 (Rat)

• Alternate Names:

  Mel91; nel (chicken)-like 2; NELL2; NEL-like 2 (chicken); NEL-like protein 2; Nel-related protein 2; NRP2; NRP2neural epidermal growth factor-like 2; protein kinase C-binding protein NELL2