详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Human USAG1 (Catalog # ) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse LRP-6 (Catalog # 2960-LR) that produces 50% of the optimal binding response is approximately 0.1-0.6 μg/mL.
- SourceE. coli-derived Phe24-Ser206, with an N-terminal Met
- Accession #
- N-terminal Sequence
AnalysisMet - Predicted Molecular Mass21 kDa
- SDS-PAGE21 kDa, reducing conditions
9008-SD | | |
Formulation Lyophilized from a 0.2 μm filtered solution in HCl. | ||
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- References:
- Laurikkala, J. et al. (2003) Dev. Biol. 264:91.
- Yanagita, M. et al. (2004) Biochem. Biophys. Res. Commun. 316:490.
- Lintern, K.B. et al. (2009) J. Biol. Chem. 284:23159.
- Yanagita, M. et al. (2006) J. Clin. Invest. 116:70.
- Ellies, D.L. et al. (2014) PLoS One 9:e96257.
- Ahn, Y. et al. (2013) Development 140:583.
- Maeda, K. et al. (2007) J. Reprod. Dev. 53:931.
- Murashima-Suginami, A. et al. (2008) Biochem. Biophys. Res. Commun. 369:1012.
- Ahn, Y. et al. (2010) Development 137:3221.
- Tanaka, M. et al. (2010) J. Clin. Invest. 120:768.
- Kassai, Y. et al. (2005) Science 309:2067.
- Long Name:Sclerostin Domain Containing Protein 1/Uterine Sensitization-associated Gene 1 Protein
- Entrez Gene IDs:25928 (Human); 66042 (Mouse); 266803 (Rat)
- Alternate Names:CDA019; cystine-knot containing secreted protein; DKFZp564D206; Ectodermal BMP inhibitor; Ectodin; sclerostin domain containing 1; sclerostin domain-containing protein 1; SOSTDC1; USAG1; USAG-1; USAG1uterine sensitization-associated protein-1; Uterine sensitization-associated gene 1 protein; WISE