详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey IgG 1 Fc is immobilized at 0.15 µg/mL (100 µL/well), the concentration of Recombinant Human Fc gamma RI/CD64 (Catalog # ) that produces 50% of the optimal binding response is 0.2-1.2 ng/mL.
- SourceHuman embryonic kidney cell, HEK293-derived Pro110-Lys333
- Accession #
- N-terminal Sequence
AnalysisPro110, and Ala-Leu-Ala-Pro110 - Predicted Molecular Mass25 kDa
- SDS-PAGE30-40 kDa, reducing conditions
9315-HG | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 1 mg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Bioactivity | When Recombinant Cynomolgus Monkey IgG1 Fc (Catalog # 9315-HG) is coated at 0.15 μg/mL, 100 μL/well, Recombinant Human Fc gamma RI/CD64 (Catalog # ) binds with an ED50 of 0.2-1.2 ng/mL. |
- References:
- Stavnezer, J. et al. (2008) Annu. Rev. Immunol. 26:261.
- Nutt, S.L. et al. (2015) Nat. Rev. Immunol. 15:160.
- Swanson, C.L. et al. (2013) Immunol. Res. 55:277.
- Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
- Long Name:Immunoglobulin G1
- Entrez Gene IDs:101867415 (Cynomolgus Monkey)
- Alternate Names:IgG1