• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 x 10    ⁴ cells/well are added to Recombinant Mouse EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog #) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED    ₅₀ for this effect is 0.4-2.4 μg/mL

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse EpCAM/TROP1 (Gln24 & Arg81-Thr266) Accession # Q99JW5	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Arg81 detected. Gln24 inferred from enzymatic pyroglutamate treatment revealing Arg25

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  55 kDa

• SDS-PAGE

  58-75 kDa and 8 kDa

Data Images

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, CD326 and TROP‑1, is a 40 kDa transmembrane glycoprotein composed of a 243 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF‑like) repeats, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In mouse, EpCAM also shares 51% aa sequence homology with Trop‑2. EpCAM is detected in basolateral cell membranes of all simple epithelia and expression has been found to increase in epithelia tissues during fetal development (1, 2). EpCAM has been shown to function as a homophilic Ca2  ⁺ independent adhesion molecule, but it does not structurally resemble any of the four major families of cell adhesion molecules (3). Homophilic adhesion via EpCAM requires the interaction of both EGF‑like repeats, with the first EGF‑like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM (4). EpCAM has been identified as a surface marker for pluripotent embryonic stem cells and is strongly associated with the maintenance of the undifferentiated state of ESCs (5). EpCAM is highly expressed on the surface of epithelial cancer cells including colon, stomach, lung, pancreas, prostate and breast (6, 7).

• References:

1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.

2. Keisuke, N. et al. (2009) PLoS One 4 :e8543

3. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.

4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.

5. González, B. et al. (2009) Stem Cells, 27: 1782.

6. Went, P. et al. (2006) Br. J. Cancer 94:128.

7. Spizzo, G.S. et al. (2002) Int. J. Cancer 98:883.

• Long Name:

  Epithelial Cell Adhesion Molecule

• Entrez Gene IDs:

  4072 (Human); 17075 (Mouse)

• Alternate Names:

  17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A