• Purity

  >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 (Catalog # ) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.

• Source

  Human embryonic kidney cell, HEK293-derived

  Human BAI2 (Phe21-Asp911 ) Accession # O60241-3	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Phe21

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  123 kDa

• SDS-PAGE

  128-200 kDa, reducing conditions

Data Images

Background: BAI2

Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).

• References:

1. Duman, J.G. et al. (2016) Neural Plast. 2016:8301737.

2. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.

3. Kee, H.J. et al. (2002) J. Cereb. Blood Flow Metab. 22:1054.

4. Daisuke, O. et al. (2010) J. Recept Signal Transduct Res 30:143.

5. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.

6. Jeong, B.C. et al. (2016) FEBS Lett 580:669.

• Long Name:

  Brain-specific Angiogenesis Inhibitor 2

• Entrez Gene IDs:

  576 (Human); 230775 (Mouse); 313058 (Rat)

• Alternate Names:

  BAI2; brain-specific angiogenesis inhibitor 2; Brain-specific angiongenesis inhibitor-2