详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeassure by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3 + T cells. The ED 50 for this effect is 0.5-2.5 μg/mL.
- SourceHuman embryonic kidney cell, HEK293-derived
Human BTNL8
(Gln18-Lys238)
Accession # Q6UX41-1IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisNo results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Trp19 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass52 kDa
- SDS-PAGE53-63 kDa, reducing conditions
9359-BT | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 200 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Bioactivity | Recombinant Human BTNL8 Fc Chimera (Catalog # 9359-BT)enhances anti-CD3-induced IFN-gamma secretion by mouse CD3+ T cells. The ED50 forthis effect is 0.5-2.5 μg/mL. |
Ag-specific IgG during the primary but not the secondary immune response (3).
- References:
- Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
- Arnett, H.A. et al. (2009) Cytokine 46:370.
- Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
- Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.
- Long Name:Butyrophilin-like 8
- Entrez Gene IDs:79908 (Human); 406160 (Rat)
- Alternate Names:BTN9.2; BTNL8; butyrophilin-like 8; butyrophilin-like protein 8; UNQ702/PRO1347