• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Meassure by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3    ⁺ T cells. The ED    ₅₀ for this effect is 0.5-2.5 μg/mL.    
   

• Source

  Human embryonic kidney cell, HEK293-derived

  Human BTNL8 (Gln18-Lys238) Accession # Q6UX41-1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Trp19

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  52 kDa

• SDS-PAGE

  53-63 kDa, reducing conditions

Data Images

Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells.   In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of  
Ag-specific IgG during the primary but not the secondary immune response (3).

• References:

1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.

2. Arnett, H.A. et al. (2009) Cytokine 46:370.

3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.

4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.

• Long Name:

  Butyrophilin-like 8

• Entrez Gene IDs:

  79908 (Human); 406160 (Rat)

• Alternate Names:

  BTN9.2; BTNL8; butyrophilin-like 8; butyrophilin-like protein 8; UNQ702/PRO1347