详细说明
- Purity>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by the ability of the immobilized protein to support the adhesion of CCD-1070Sk human normal skin fibroblasts. The ED 50 for this effect is 0.1-0.4 μg/mL
- SourceChinese Hamster Ovary cell line, CHO-derived Arg23-Lys1403, with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisLeu28 - Predicted Molecular Mass150 kDa
- SDS-PAGE135-230 kDa, reducing conditions
9335-SN | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Bioactivity | Immobilized Recombinant Mouse SNED1 (Catalog #9335-SN) supports the adhesion of CCD-1070Sk human normal skinfibroblasts The ED50 for this effect is 0.1-0.4 μg/mL. |
- References:
- Leimeister, C. et al. (2004) Developmental Dynamics. 230:371.
- Ueno, H. et al. (2003) Nature Immunology. 4:457.
- Naba, A. et al. (2014) eLife. 3:e01308.
- Longati, P. et al. (2013) BMC Cancer. 13:95.
- Long Name:Sushi, Nidogen and EGF-like Domain-containing Protein
- Entrez Gene IDs:25992 (Human); 208777 (Mouse); 316638 (Rat)
- Alternate Names:DKFZp586B2420; FLJ00133,6720455I24Rik homolog; insulin responsive sequence DNA binding protein-1; Insulin-responsive sequence DNA-binding protein 1; IRE-BP1; SNED1; Snep; SST3; sushi, nidogen and EGF-like domain-containing protein 1; sushi, nidogen and EGF-like domains 1