详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED 50 for this effect is 0.15-0.9 μg/mL
- SourceHuman embryonic kidney cell, HEK293-derived Asp19-Thr256, with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisAsp19 - Predicted Molecular Mass27 kDa
- SDS-PAGE54 - 70 kDa, reducing conditions
9327-B7 | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 200 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Bioactivity | Recombinant Cynomolgus Monkey B7-H2 (Catalog # 9327-B7)co-stimulates IL-4 secretion by D10.G4.1 mouse helper T cells in the presenceof anti-CD3 antibody. The ED50 for this effect is 0.15-0.9 μg/mL. |
(2-6). B7-H2 binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation (2, 4, 7). Mouse and human B7-H2 exhibit cross-species binding to ICOS (3, 6). The B7-H2 interaction with ICOS is co-stimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (Tfh) and germinal centers (2-4, 8, 9). In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate both human and mouse naïve T cells and regulatory T cells (Treg) (6). B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung (8, 10, 11). Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity (5, 12, 13). In contrast, its ligation of ICOS on Tfh cells can increase the severity of autoimmune symptoms (9). A soluble form of human B7-H2 is elevated in the circulation of patients with active systemic lupus erythematosus (14).In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones (15). B7-H2 and ICOS expressed on ILC2 cells. B7-H2/ICOS interaction promoted cytokine production and survival in ILC2 cells through STAT5, suggesting that B7-H2/ICOS signaling pathway is critically involved in ILC2 function and homeostasis (16).
- References:
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Wang, S. et al. (2000) Blood 96:2808.
- Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
- Faget, J. et al. (2012) Cancer Res. 72:6130.
- Yao, S. et al. (2011) Immunity 32:729.
- Watanabe, M. et al. (2008) J. Immunol. 180:5222.
- Wong, S.-C. et al. (2003) Blood 102:1381.
- Hu, Y.-L. et al. (2009) J. Immunol. 182:1421.
- Kadkhoda, K. et al. (2010) J. Immunol. 184:3780.
- Kadkhoda, K. et al. (2011) Int. Immunol. 23:239.
- Gajewska, B.U. et al. (2005) J. Immunol. 174:3000.
- Akbari, O. et al. (2002) Nat. Med. 8:1024.
- Her, M. et al. (2009) Lupus 18:501.
- Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.
- Maazi H. et al. (2015) Immunity.42:538.
- Long Name:B7 Homolog 2
- Entrez Gene IDs:23308 (Human); 50723 (Mouse); 102132485 (Cynomolgus Monkey)
- Alternate Names:B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand