Recombinant Human PSG-6 Protein, CF 50 UG

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Recombinant Human PSG-6 Protein, CF 50 UG信息二维码

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3-Amino-5-methoxycarbonylphenylboronic acid, pinacol ester  1 g 2,3-Dichloro-6-(trifluoromethyl)benzyl bromide  1 g 1-(4-Fluorophenyl)-5-methoxycarbonyl-2(1H)-pyridinone  1 g N,N-Diethyl-cyclohexane-1,4-diamine  1 g N-Methyl-DL-leucine hydrochloride  5 g N-(2-Chloroethyl) 3-boronobenzamide  5 g

产品介绍

    基本参数

    详细说明

    • Purity
      >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    • Endotoxin Level
      <0.10 EU per 1 μg of the protein by the LAL method.
    • Activity
      Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED 50 for this effect is 0.9-4.5 μg/mL.
    • Source
      Human embryonic kidney cell, HEK293-derived Gln35-His424, with a C-terminal 6-His tag
    • Accession #
    • N-terminal Sequence
      Analysis
      No results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36
    • Predicted Molecular Mass
      45 kDa
    • SDS-PAGE
      62-73 kDa, reducing conditions
    9318-P6
     
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
    Reconstitution Reconstitute at 500 μg/mL in PBS.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Data Images
    Binding Activity

    Recombinant Human PSG-6 (Catalog # 9318-P6) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.
    Background: PSG-6
    Pregnancy-specific glycoprotein 6 (PSG-6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily (1). Mature human PSG-6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa
    C-terminal tail (2, 3). The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins (4, 5). Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG-6 is predominantly expressed in placenta and more weakly in granulocytes (3). It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1 (6).
    • References:
      1. Kuespert, K. et al. (2006) Curr. Opin. Cell Biol. 18:565.
      2. Zimmermann, W. et al. (1989) Biochem. Biophys. Res. Commun. 163:1197.
      3. Barnett, T.R. et al. (1990) Biochemistry 29:10213.
      4. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
      5. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
      6. Snyder, S.K. et al. (2001) Am. J. Reprod. Immunol. 45:205.
    • Long Name:
      Pregnancy-specific beta-1-glycoprotein 6
    • Entrez Gene IDs:
      5675 (Human)
    • Alternate Names:
      CGM3; pregnancy specific beta-1-glycoprotein 6; Pregnancy-specific beta-1-glycoprotein 10; Pregnancy-specific beta-1-glycoprotein 12; pregnancy-specific beta-1-glycoprotein 6; Pregnancy-specific glycoprotein 10; Pregnancy-specific glycoprotein 12; Pregnancy-specific glycoprotein 6; PS-beta-G-10; PS-beta-G-12; PS-beta-G-6; PSBG-10; PSBG-12; PSBG-6; PSG10; PSG12; PSG6; PSG-6; PSGGB
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