• Purity

  >70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human C1qTNF10/C1qL2 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Protein Fc Chimera (Catalog # ) that produces 50% of the optimal binding response is approximately 0.075-0.45 μg/mL.

• Source

  E. coli-derived Phe154-Asp287, with an N-terminal Met and 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Structure / Form

  Noncovalently-linked homotrimer

• Predicted Molecular Mass

  16 kDa

• SDS-PAGE

  14 kDa, reducing conditions

Data Images

Background: C1qTNF10/C1qL2

C1qTNF10 (CTRP10), also known as C1qL2, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF10 contains a collagen-like region and one C1q-like domain. It can form heterotrimers and higher order disulfide linked oligomers with C1qTNF1, C1qTNF11/C1qL4, and C1qTNF13/C1qL3 (3-6). Within the C1q-like domain, human C1qTNF10 shares 100% aa sequence identity with mouse and rat C1qTNF10. Similarly to C1qTNF11, C1qTNF13, and C1qTNF14/C1qL1, C1qTNF10 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (7). It is produced by mossy fibers in the hippocampus where it enhances the postsynaptic clustering of kainate-type glutamate receptors (KAR) on CA3 pyramidal neurons (8). This function is enhanced by the binding of C1qTNF10 to the KAR subunits GluK2 and GluK4 as well as to Neurexin-3 beta containing the exon 5b splice site insertion (8).

• References:

1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.

2. Yuzaki, M. (2001) Eur. J. Neurosci. 32:191.

3. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.

4. Wei, Z. et al. (2011) J. Biol. Chem. 286:15652.

5. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.

6. Wong, G.W. et al. (2008) Biochem. J. 416:161.

7. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.

8. Matsuda, K. et al. (2016) Neuron 90:752.

• Long Name:

  C1q And Tumor Necrosis Factor Related Protein 10

• Entrez Gene IDs:

  165257 (Human); 226359 (Mouse)

• Alternate Names:

  C1q and tumor necrosis factor related protein 10; C1q-domain containing protein; C1qL2; C1qTNF10; complement C1q-like protein 2; complement component 1, q subcomponent-like 2; CTRP10