详细说明
- Purity>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED 50 of 150-900 ng/mL.
- SourceChinese Hamster Ovary cell line, CHO-derived
Human Siglec-15
(Phe20-Thr263)
Accession # Q6ZMC9IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisPhe20 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass53 kDa
- SDS-PAGE53-64 kDa, reducing conditions
9227-SL | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose. | ||
Reconstitution Reconstitute at 200 μg/mL in water. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Bioactivity | When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto aStreptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera (Catalog # 9227-SL) bindswith an ED50 of 150-900 ng/mL. |
- References:
- Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
- Angata, T. et al. (2007) Glycobiology 17:838.
- Takamiya, R. et al. (2013) Glycobiology 23:178.
- Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
- Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
- Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
- Long Name:Sialic Acid Binding Ig-like Lectin 15
- Entrez Gene IDs:284266 (Human); 620235 (Mouse); 498888 (Rat)
- Alternate Names:CD33 antigen-like 3SIGLEC-15; CD33 molecule-like 3; CD33L3; HsT1361; sialic acid binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15