• Purity

  >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. The ED    ₅₀ for this effect is 0.4-2.4 μg/mL.    
   

• Source

  Human embryonic kidney cell, HEK293-derived

  Human Desmoglein-4 (Glu24-Ala631) Accession # Q86SJ6	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu24

• Structure / Form

  Disulfide-liked homodimer

• Predicted Molecular Mass

  94 kDa

• SDS-PAGE

  95-115 kDa, reducing conditions

Data Images

Background: Desmoglein-4

Desmoglein-4 is an approximately 140 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. Human Desmoglein-4 is synthesized with a 26 amino acid (aa) propeptide followed by a 582 aa extracellular domain (ECD) with four tandem cadherin-like domains, a 21 aa transmembrane segment, and a 388 aa cytoplasmic domain (1, 2). Within the ECD, human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4. Alternative splicing generates additional isoform with a substitution in the cytoplasmic domain. Desmoglein-4 is the principal cadherin of desmosomes which form adhesive contacts between epithelial cells (2, 3). It is expressed in hair follicles and differentiated keratinocytes in suprabasal layers of the skin and plays a role in keratinocyte differentiation and adhesion (2, 3). Mutations in human Desmoglein-4 are associated with the development of monilethrix autosomal recessive hypotrichosis (LAH) (2, 4-8). Autoantibodies directed against Desmoglein-3 in the blistering skin disorder pemphigus vulgaris can cross-react with Desmoglein-4 (2, 9, 10).

• References:

1. Whittock, N.V. and C. Bower (2003) J. Invest. Dermatol. 120:523.

2. Kljuic, A. et al. (2003) Cell 113:249.

3. Bazzi, H. et al. (2006) Differentiation 74:129.

4. Bazzi, H. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:222.

5. Schaffer, J.V. et al. (2006) J. Invest. Dermatol. 126:1286.

6. Shimomura, Y. et al. (2006) J. Invest. Dermatol. 126:1281.

7. Zlotogorski, A. et al. (2006) J. Invest. Dermatol. 126:1292.

8. Kato, M. et al. (2015) J. Invest. Dermatol. 135:1253.

9. Nagasaka, T. et al. (2004) J. Clin. Invest. 114:1484.

10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.

• Entrez Gene IDs:

  147409 (Human); 16769 (Mouse); 291754 (Rat)

• Alternate Names:

  Cad13; Cadherin family member 13; CDGF13; CDHF13; CDHF13CDH family member 13; desmoglein 4; Desmoglein4; Desmoglein-4; DSG4; LAHdesmoglein-4