• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 x 10    ⁴ cells/well are added to Recombinant Human EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog #) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED    ₅₀ for this effect is 0.4-2.4 μg/mL.

• Source

  Human embryonic kidney cell, HEK293-derived Gln24-Lys265, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained. Gln24 inferred from enzymatic pyroglutamate treatment revealing Glu25

• Predicted Molecular Mass

  28 kDa

• SDS-PAGE

  30-42 kDa, reducing conditions

Data Images

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain (1). Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16).

• References:

1. Strnad, J. et al. (1989) Cancer Res. 49:314.

2. Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.

3. Ng, V.Y. et al. (2010) Stem Cells 28:29.

4. Nelson, A.J. et al. (1996) Eur. J. Immunol. 26:401.

5. Borkowski, T.A. et al. (1996) Eur. J. Immunol. 26:110.

6. de Boer, C.J. et al. (1999) J. Pathol. 188:201.

7. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.

8. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.

9. Nubel, T. et al. (2009) Mol. Cancer Res. 7:285.

10. Kuhn, S. et al. (2007) Mol. Cancer Res. 5:553.

11. Schmidt, D.S. et al. (2004) Exp. Cell Res. 297:329.

12. Litvinov, S.V. et al. (1997) J. Cell Biol. 139:1337.

13. Gaiser, M.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E889.

14. Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.

15. Schon, M.P. et al. (1993) Int. J. Cancer 55:988.

16. Maetzel, D. et al. (2009) Nat. Cell Biol. 11:162.

• Long Name:

  Epithelial Cell Adhesion Molecule

• Entrez Gene IDs:

  4072 (Human); 17075 (Mouse)

• Alternate Names:

  17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A