• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17RC Isoform 1 Fc Chimera (Catalog # 9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # ) binds with an ED    ₅₀ = 0.1-0.6 ng/mL.

• Source

  Human embryonic kidney cell, HEK293-derived

  Human IL-17 RC Isoform 1 (Leu21-His465) Accession # NP_703190.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Leu21

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  76 kDa

• SDS-PAGE

  95-113 kDa, reducing conditions

Data Images

Background: IL-17 RC

IL-17 receptor C (IL-17RC; also known as IL-17RL and IL-17Rhom) is an 85-110 kDa member of the IL-17 receptor family. IL-17 plays a key role in the development of autoimmunity and inflammation (1, 2). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on neutrophils, endothelial cells, chondrocytes and osteoblasts, breast and prostatic epithelium, fibroblasts, renal tubular epithelium, and skeletal muscle cells (3-5). Mature human IL-17 RC consists of a 518 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region (6). It is estimated that there are over 90 alternative splice forms, with transmembrane-containing isoforms predominating (4, 7). This recombinant protein corresponds to IL-17 RC isoform 1, the canonical form. Within the ECD, IL-17 RC isoform 1 shares 69% aa sequence identity with mouse IL-17 RC. IL-17 RC is the cognate receptor for IL-17F, and it binds IL-17A with similar affinity (8). IL-17 RC associates wiith IL-17 RA to form a receptor complex for both IL-17A and IL-17F (8-11).

• References:

1. Song, X. and Y. Qian (2013) Cytokine 62:175.

2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.

3. Taylor, P.R. et al. (2014) Nat. Immunol. 15:143.

4. You, Z. et al. (2007) Neoplasia 9:464.

5. Kokubu, T. et al. (2008) J. Hictochem. Cytochem. 56:89.

6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.

7. Haudenschild, D.R. et al. (2006) Prostate 66:1268.

8. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.

9. Toy, D. et al. (2006) J. Immunol. 177:36.

10. Kramer, J.M. et al. (2006) J. Immunol. 176:711.

11. Kramer, J.M. et al. (2007) J. Immunol. 179:6379.

• Long Name:

  Interleukin 17 Receptor C, Transcript Variant 3

• Entrez Gene IDs:

  84818 (Human); 171095 (Mouse)

• Alternate Names:

  FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14