For the differentiation of Human Regulatory T (Treg) Cells from a preparation of naïve CD4⁺ T cells.

Why Differentiate Treg Cells In Vitro?

Forkhead Box P3 (FoxP3)⁺ regulatory T (Treg) cells are a suppressive subset of CD4⁺ T cells that function to antagonize immune responses. Treg cells have the capacity to prevent potentially damaging autoimmune and protective immune responses, so the number of Treg cells is a crucial determinant of the regulatory burden on the immune system. In vitro differentiation of Treg cells from the larger naïve CD4⁺ T cell population provides increased numbers of Treg cells to facilitate downstream research.

This kit contains the following optimized proteins and reagents to drive efficient differentiation of naïve CD4⁺ T cells into FoxP3⁺CD25⁺ Treg cells.

• Mouse Anti-Human CD3 Antibody
• Human Treg Reagent 1
• Human Treg Reagent 2
• Human Treg Reagent 3
• Human Treg Reagent 4
• Reconstitution Buffer 1
• Reconstitution Buffer 2
• Wash Buffer (20X)

Provides sufficient reagents for the differentiation of two 24-well plates.

Stability and Storage

Store the unopened kit at 2-8 ^°C. After opening the kit, the Mouse Anti-Human CD3 Antibody and Human Treg Reagents 1-4 may be stored at 2-8 ^°C under sterile conditions for up to 30 days or at -20 ^°C to -70 ^°C in a manual defrost freezer for up to 3 months. Reconstitution Buffer 1, Reconstitution Buffer 2, and 20X Wash Buffer may be stored under sterile conditions for up to 3 months at 2-8 ^°C. Storage conditions are valid if used within the expiration date of the kit.