For the differentiation of regulatory T Cells (Tregs) from a preparation of naïve CD4⁺ T cells.

Why Differentiate Treg Cells In Vitro?

Forkhead Box P3 (FoxP3)⁺ regulatory T (Treg) cells are a suppressive subset of CD4⁺ T cells that function to antagonize immune responses. Treg cells have the capacity to prevent potentially damaging autoimmune and protective immune responses, so the number of Treg cells is a crucial determinant of the regulatory burden on the immune system. In vitro differentiation of Treg cells from the larger naïve CD4⁺ T cell population provides increased numbers of Treg cells to facilitate downstream research.

This kit contains the following optimized proteins and reagents to drive efficient differentiation of naïve CD4⁺ T cells into FoxP3⁺CD25⁺ Treg cells.

• Hamster Anti-Mouse CD3 Antibody
• Mouse Treg Reagent 1
• Mouse Treg Reagent 2
• Mouse Treg Reagent 3
• Reconstitution Buffer 1
• Reconstitution Buffer 2
• Wash Buffer (20X)

Stability and Storage

Store the unopened kit at -20 °C. After opening the kit, the Human Anti-Mouse CD3 Antibody, Mouse Treg Reagent 1, and Mouse Treg Reagent 2 may be stored at 2-8 °C under sterile conditions for up to 30 days or at -20 °C to -70 °C in a manual defrost freezer for up to 3 months. Mouse Treg Reagent 3 may be stored at -20 °C to -70 °C in a manual defrost freezer for up to 3 months. Reconstitution Buffer 1, Reconstitution Buffer 2, and 20X Wash Buffer may be stored under sterile conditions for up to 3 months at 2 to 8 °C. Storage conditions are valid if used within the expiration date of the kit.