• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED    ₅₀ for this effect is 20-120 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # ) .

• Source

  Mouse myeloma cell line, NS0-derived Thr18-Ala247, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Thr18

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  27 kDa

• SDS-PAGE

  26-43 kDa, reducing conditions

Background: Stanniocalcin 1/STC-1

Stanniocalcin 1 (STC-1) is a homodimeric glycoprotein hormone that is involved in calcium and phosphate homeostasis. It was originally identified in bony fishes, where elevation of calcium in serum causes the release of STC from the endocrine glands called the corpuscles of Stannius (1-3). In contrast to fish where expression is mainly in the corpuscles of Stannius, the STC-1 gene in mammals is widely expressed in tissues including thyroid, ovary, prostate, adrenal gland, muscle, intestine, kidney, heart and lung (4). Human STC-1 protein consists of a 16 amino acid (aa) propeptide region and a 214 aa STC-1 chain. The amino acid sequence of human mature STC-1 is 36% identical to that of human STC-2. STC-1 is highly homologous with mouse, sharing 95% amino acid identity. STC-1 inhibits the breakdown of PAPP-A (5), protects cancer cells from apoptosis (4), reduces tumor size of liver cancers (6), promotes osteoblast differentiation (7) and inhibits longitudinal bone growth directly at the growth plate (8). It is also a biomarker of brain and lung cancer progression (7, 8).

• References:

1. Yoshiko, Y. and J.E. Aubin. (2004) Peptides 25:1663.

2. Wendelaar Bonga S.E. and P.K. Pang (1991) Int. Rev. Cytol. 128:139.

3. Wascher, R.A. et al. (2003) Clin. Cancer Res. 9:1427.

4. ­Ohkouchi, S. et al. (2015) Clin. Med. Insights Circ. Respir. Pulm. Med. 9:91.

5. Kloverpris S, et al. (2015) J Biol Chem. 290:21915.

6. Yeung BH, et al. (2015) PLoS One. 10:e0139977.

7. Su J, et al. (2015) Tumour Biol. 36:6333.

8. Du YZ, et al. (2016) Tumour Biol. 37:5633.

• Entrez Gene IDs:

  6781 (Human)

• Alternate Names:

  Stanniocalcin 1; stanniocalcin-1; STC1; STC-1; STCSTC-1